[3.10] - 2022-12-21
Enhancements & fixes
- Bump minimum Nextflow version from
21.10.3
->22.10.1
- Updated pipeline template to nf-core/tools 2.7.2
- [#729] - Add 'auto' option to samplesheet to automatically detect strandedness for samples
- [#889] - Document valid options for
--genome
parameter - [#891] - Skip MarkDuplicates when UMIs are used
- [#896] - Remove
copyTo
call for iGenomes README - [#897] - Use
--skip_preseq
by default - [#898] - Documentation on salmon decoy-aware index creation, gcbias and seqbias
- [#900] - Add
--recursive
option tofastq_dir_to_samplesheet.py
script - [#902] -
check_samplesheet.py
script doesn't output optional columns in samplesheet - [#907] - Add
--extra_star_align_args
and--extra_salmon_quant_args
parameter - [#912] - Add UMI deduplication before quantification in tube map
Parameters
Old parameter | New parameter |
---|---|
--enable_conda
| |
--extra_star_align_args
| |
--extra_salmon_quant_args
|
NB: Parameter has been updated if both old and new parameter information is present.
NB: Parameter has been added if just the new parameter information is present.
NB: Parameter has been removed if new parameter information isn't present.
Software dependencies
Note, since the pipeline is now using Nextflow DSL2, each process will be run with its own Biocontainer. This means that on occasion it is entirely possible for the pipeline to be using different versions of the same tool. However, the overall software dependency changes compared to the last release have been listed below for reference.
Dependency | Old version | New version |
---|---|---|
bbmap
| 38.93 | 39.01 |
bioconductor-dupradar
| 1.18.0 | 1.28.0 |
bioconductor-summarizedexperiment
| 1.20.0 | 1.24.0 |
bioconductor-tximeta
| 1.8.0 | 1.12.0 |
fq
| 0.9.1 | |
salmon
| 1.5.2 | 1.9.0 |
samtools
| 1.15.1 | 1.16.1 |
NB: Dependency has been updated if both old and new version information is present.
NB: Dependency has been added if just the new version information is present.
NB: Dependency has been removed if version information isn't present.